The adoption of arrayCGH for the investigation of genomic copy number imbalance in patients presenting with constitutional genetic disorders has changed the nature of FISH (fluorescent in situ hybridization). Previously a small library of FISH probes, in combination with karyotyping, were sufficient to investigate a limited number of well characterized syndromes. By contrast arrayCGH offers a much higher resolution screen able to identify numerous regions of copy number variation (CNVs) at loci from across the genome. Current clinical practice is to confirm all CNVs with a suspected phenotypic association. BlueFish Tilepath probes enable these requirements to be met by offering clinical quality FISH probes spanning the entire genome.
- Coverage of the entire genome. The BlueFish Tilepath library contains over 26,000 probes; the largest of its kind. The high density of BlueFish probes ensures that the correct probe is available in the region of interest.
- The standard. BlueFish probes are supplied under a commercial licence from the Roswell Park Cancer Institute. The familiar RP nomenclature is widely referenced in the literature and all the major genome browsers enabling the best probe for any application to be easily identified.
- On-line service. BlueGnome FISH probes may be ordered in bulk and then called off as required. Comprehensive online ordering facilities enable clones to be selected on the basis of their RP nomenclature, cytogenetic band or midpoint position. A choice of labels, SpectrumGreen™, SpectrumOrange™ or SpectrumRed™ range equivalents, are selected after which the required probe is submitted for production, with delivery typically in 14 days.
- Easier interpretation. Direct labelling and simple protocols ensure high signal and low background for rapid, sensitive and specific confirmation of CNVs in metaphase chromosomes.
All BlueFish Tilepath probes undergo the same rigorous, quality control and production procedure as those used on BlueGnome's CytoChip. Note that the genome is known to contain significant regions of cross homology which will be reflected in the Tilepath probes, this should be taken into consideration when selecting probes.
To evaluate BlueFish in your laboratory please contact us for two complimentary probes.
